Slender Layer Chromatography Lab Report


The purpose of this experiment is always to analyze mixtures of compounds prior to, during and after a separation structure. This try things out also permits monitoring reactions of organic and natural molecules, and determines the identity of a mixture of compounds.


[1] SOLVENTS| a. Hexane1Molecular Molarity: 86. 18 g/molBoiling Level: 69 ºCMelting Point: -95ºCDensity: 0. 659 g/mL for 25ºCWater Solubility: Insoluble in waterColor/Texture: Colorless/LiquidHazardous Information: Could potentially cause irritation for the eyes, epidermis and breathing. May cause drowsiness and/or dizziness. Suspected of damaging fertility or unborn child. May cause damage to internal organs. Toxic to aquatic lifestyle. Highly flammable liquid and vapor. May be fatal in the event swallowed. Framework: CH3(CH2)4CH3| | b. Methanol2Molecular Molarity: thirty-two. 04 g/molBoiling Point: sixty four. 7ºCMelting Point: -98ºCDensity: 0. 791 g/mL at 25ºCWater Solubility: Entirely miscibleColor/Texture: Colorless/LiquidHazardous Information: Very flammable water and vapour. Toxic in the event swallowed or in contact with skin area. Causes skin area and eye irritation. Causes injury to organs. Structure: CH3OH| | c. Methylene Chloride3Molecular Molarity: 84. 93 g/molBoiling Point: 39. 8-40. 0ºCMelting Level: -97ºCDensity: 1 ) 325 g/mL at 25ºCWater Solubility: Slightly soluble in waterColor/Texture: Colorless/LiquidHazardous Information: Harmful if swallowed. Causes skin and eye diseases. Suspected of causing malignancy. Structure: CH2Cl2| 2 . Feasible Components of Mixture| a. Biphenyl4Molecular Molarity: 154. 21 g/molBoiling Point: 255ºCMelting Point: 68-70ºCDensity: 0. 992 g/cm3Water Solubility: 0. 0075 g/L in 15ºCColor/Texture: Light yellow/CrystallineHazardous Information: May be hazardous if swallowed. Causes skin and critical eye irritation. May cause respiratory irritation. Very toxic to aquatic life. Composition: C12H10| | b. Benzophenone5Molecular Molarity: 182. 22 g/molBoiling Point: 305ºCMelting Point: 47-51ºCDensity: N/AWater Solubility: N/AColor/Texture: White/CrystallineHazardous Information: Might be harmful in the event that inhaled. Could potentially cause respiratory tract discomfort. May be damaging if assimilated through skin area. May cause epidermis and eye irritation. May be harmful if ingested. Structure: C13H10O| | c. Benzhydrol6Molecular Molarity: 184. 23 g/molBoiling Level: 297-298ºCMelting Level: 65-67ºCDensity: N/AWater Solubility: N/AColor/Texture: Crystalline/whiteHazardous Data: May be damaging if swallowed. Causes epidermis and critical eye irritation. Could cause respiratory tract discomfort. Structure: C13H12O| PROCEDURE

The experiment started with three elution rooms prepared using 150 milliliters and two hundred fifity mL beakers. One filtering paper was placed inside each beaker. Enough hexane was added to the bottom of the first beaker, methylene chloride in the second beaker and methanol for the third beaker. The beakers were designated with the id of the solvents. The filtration system papers in the beakers were wet making use of the respective solvents then protected with aluminum foil. 3 small TLC plates were obtained, cautiously held on the edges to stop contamination coming from fingers, and placed under a UV light to confirm that there are not any impurity places on them. By approximate 4mm above the edge of the bottom of the TLC plates, a line was drawn throughout each 1 with a great " X” in the middle of the queue in each one indicating an origin. The discs were creatively checked to make certain the silica gel had not been disturbed. A single spot with the known mixture was put on the beginnings using a capillary tube. The known combination contains: Biphenyl, Benzophenone, and Benzhydrol. The solvent was set aside to evaporate then placed under the UV light once again to verify if a dark spot is present in every plate. The plates had been then placed in each of the beakers prepared previously; one menu in the hexane eluting holding chamber, another dish in the methylene chloride eluting chamber as well as the last dish in the methanol eluting chamber. The beakers were shut down loosely...

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2MSDS. Representative. no . 34860. N. l.: n. l., n. m. Sigma Aldrich. Sigma Aldrich. Web. 13 Oct. 2012..

3MSDS. Rep. no . 270997. N. s.: n. g., n. g. Sigma Aldrich. Sigma Aldrich. Web. 16 Oct. 2012..

4MSDS. Associate. no . W312908. N. p.: n. g., n. d. Sigma Aldrich. Sigma Aldrich. Web. 16 Oct. 2012..

5MSDS. Representative. no . 427551. N. l.: n. p., n. g. Sigma Aldrich. Sigma Aldrich. Web. 14 Oct. 2012..

6MSDS. Representative. no . B4856. N. p.: n. s., n. g. Sigma Aldrich. Sigma Aldrich. Web. 14 Oct. 2012..

Benzhydrol can be synthesized from Benzophenone via a reduction reaction. Samples of the reaction mixture will be taken in several times through the reaction (start, 50% of the way with the end from the reaction time). Draw the anticipated outcomes (spots) intended for silica gel TLC china at the specific reaction (not elution) instances (assume the very best solvent applied above):

For start (no reaction) At 50 % completion At 100 % completion



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